The possible transitive sense, ‘to produce a sound from a musical instrument’ only seems to be attested in a figurative usage. Moreover, in this single v.a. attestation the verb could also be interpreted as being used impersonally (‘une tel note’ being the subject rather than the direct object).

For more information on the medieval practice of polyphony or playing ‘in organum’, see Horn ii p.156 and the OED entry for organum n.1 sense 4.